3CX6

Crystal Structure of PDZRhoGEF rgRGS Domain in a Complex with Galpha-13 Bound to GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97921
Spacegroup name	P 21 21 21
Unit cell lengths	58.036, 66.872, 150.902
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	75.000 - 2.500
R-factor	0.266
Rwork	0.227
R-free	0.28500
Starting model (for MR)	1zcb 1htj
RMSD bond length	0.008
RMSD bond angle	1.293
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.000	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	20558
<I/σ(I)>	17	1.5
Completeness [%]	98.0	83.3
Redundancy	6.4	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	22-27 % polyethylene glycol 4000 and 100 mM Na+ HEPES, pH 7.2-8.1, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K