3CX5
Structure of complex III with bound cytochrome c in reduced state and definition of a minimal core interface for electron transfer.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-11 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 145.120, 165.090, 194.370 |
Unit cell angles | 90.00, 104.09, 90.00 |
Refinement procedure
Resolution | 18.990 - 1.900 |
R-factor | 0.245 |
Rwork | 0.245 |
R-free | 0.26300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB entries 1KB9 and 1YCC |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.000 | 2.200 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 660107 | |
<I/σ(I)> | 7.9 | 2.43 |
Completeness [%] | 89.2 | 79 |
Redundancy | 3 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microbatch (paraffin oil) | 7.5 | 277 | 1M Sucrose, 10% DMSO, 20mM Tris pH 7.5, 80mM NaCl, 0.05 % UM, 1 M stigmatellin, 5% PEG 4000, Microbatch (paraffin oil), temperature 277K |