3CX2

Crystal structure of the C1 domain of cardiac isoform of myosin binding protein-C at 1.3A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX10.1
Synchrotron site	SRS
Beamline	PX10.1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-02-23
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.283
Spacegroup name	I 41
Unit cell lengths	48.848, 48.848, 95.132
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.300
R-factor	0.164
R-free	0.20900
Structure solution method	MIR
Starting model (for MR)	2v6h
RMSD bond length	0.010
RMSD bond angle	0.027
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	REFMAC
Refinement software	SHELX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.240
High resolution limit [Å]	1.200	1.200
Rmerge	0.046	0.642
Number of reflections	26342
<I/σ(I)>	14	1.3
Completeness [%]	88.4	33.3
Redundancy	3.9	1.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.9	293	Crystals were grown at 20 C using the hanging drop method with a 2 microlitre drop containing a 1:1 mixture of protein solution and crystallising solution consisting of 8.5% PEG 3350 and 0.05M Hepes pH 6.8 over 500 microlitres of crystallising solution in the reservoir, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K