3CW2
Crystal structure of the intact archaeal translation initiation factor 2 from Sulfolobus solfataricus .
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X12 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-02-16 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.84230 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 79.200, 162.920, 161.280 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.912 - 2.800 |
Rwork | 0.225 |
R-free | 0.27600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2plf |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.913 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.069 | |
Number of reflections | 95812 | |
<I/σ(I)> | 10.71 | |
Completeness [%] | 95.3 | 90.9 |
Redundancy | 3.16 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.1M Tris HCl, 0.72M sodium formate, 9% PEG 8000, 9% PEG 1000, 1% monomethyl ether polyethylene glycol 5000 , pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |