3CW1
Crystal Structure of Human Spliceosomal U1 snRNP
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-09 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.2557,1.2511 |
Spacegroup name | P 1 |
Unit cell lengths | 126.471, 127.075, 152.024 |
Unit cell angles | 95.42, 105.92, 101.80 |
Refinement procedure
Resolution | 123.091 - 5.493 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.4) |
Phasing software | SHARP |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 123.091 | 5.780 |
High resolution limit [Å] | 5.493 | 5.493 |
Rmerge | 0.082 | 0.691 |
Total number of observations | 31601 | |
Number of reflections | 28397 | |
<I/σ(I)> | 12.9 | 1.1 |
Completeness [%] | 99.1 | 98.3 |
Redundancy | 7.9 | 7.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 277 | 38-40% MPD, 0.3M KCl, 0.1M MES.KOH, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 277 | 38-40% MPD, 0.3M KCl, 0.1M MES.KOH, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 277 | 38-40% MPD, 0.3M KCl, 0.1M MES.KOH, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 277 | 38-40% MPD, 0.3M KCl, 0.1M MES.KOH, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MPD | ||
2 | 1 | 1 | KCl | ||
3 | 1 | 1 | MES.KOH | ||
4 | 1 | 2 | MPD | ||
5 | 1 | 2 | KCl | ||
6 | 1 | 2 | MES.KOH |