3CW1
Crystal Structure of Human Spliceosomal U1 snRNP
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-09 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.2557,1.2511 |
| Spacegroup name | P 1 |
| Unit cell lengths | 126.471, 127.075, 152.024 |
| Unit cell angles | 95.42, 105.92, 101.80 |
Refinement procedure
| Resolution | 123.091 - 5.493 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.4) |
| Phasing software | SHARP |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 123.091 | 5.780 |
| High resolution limit [Å] | 5.493 | 5.493 |
| Rmerge | 0.082 | 0.691 |
| Total number of observations | 31601 | |
| Number of reflections | 28397 | |
| <I/σ(I)> | 12.9 | 1.1 |
| Completeness [%] | 99.1 | 98.3 |
| Redundancy | 7.9 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 277 | 38-40% MPD, 0.3M KCl, 0.1M MES.KOH, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 277 | 38-40% MPD, 0.3M KCl, 0.1M MES.KOH, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 277 | 38-40% MPD, 0.3M KCl, 0.1M MES.KOH, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 277 | 38-40% MPD, 0.3M KCl, 0.1M MES.KOH, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | KCl | ||
| 3 | 1 | 1 | MES.KOH | ||
| 4 | 1 | 2 | MPD | ||
| 5 | 1 | 2 | KCl | ||
| 6 | 1 | 2 | MES.KOH |
