3CVV
Drosophila melanogaster (6-4) photolyase bound to ds DNA with a T-T (6-4) photolesion and F0 cofactor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Detector technology | CCD |
Collection date | 2007-12-06 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97925 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 84.590, 91.070, 88.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.720 - 2.100 |
R-factor | 0.16844 |
Rwork | 0.166 |
R-free | 0.21254 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.009 |
RMSD bond angle | 1.393 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.082 | 0.362 |
Number of reflections | 39271 | |
<I/σ(I)> | 15.2 | 5 |
Completeness [%] | 97.0 | 93 |
Redundancy | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 293 | 15-25% PEG1500, 0.1 M MIB buffer, pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MIB buffer | ||
2 | 1 | 2 | MIB buffer | ||
3 | 1 | 2 | PEG1500 |