3CVM
High resolution structure of a stable Plasminogen activator inhibitor type-1 in its protease cleaved form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-02 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.603, 104.014, 76.426 |
| Unit cell angles | 90.00, 101.93, 90.00 |
Refinement procedure
| Resolution | 19.635 - 2.020 |
| R-factor | 0.229 |
| Rwork | 0.228 |
| R-free | 0.28200 |
| Starting model (for MR) | 9pai |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.813 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.100 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Rmerge | 0.127 | 0.551 |
| Number of reflections | 75140 | 8251 |
| <I/σ(I)> | 9.31 | 2.5 |
| Completeness [%] | 97.8 | 98.6 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1M Tris, 4.5M Ammonium Acetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
