3CUE
Crystal structure of a TRAPP subassembly activating the Rab Ypt1p
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9795, 0.9537 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 115.120, 115.400, 290.070 |
Unit cell angles | 90.00, 90.28, 90.00 |
Refinement procedure
Resolution | 25.000 - 3.700 |
R-factor | 0.265 |
Rwork | 0.265 |
R-free | 0.29900 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.840 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.830 |
High resolution limit [Å] | 3.700 | 3.700 |
Rmerge | 0.080 | 0.368 |
Number of reflections | 81195 | |
<I/σ(I)> | 11.6 | 2.2 |
Completeness [%] | 98.5 | 91.4 |
Redundancy | 3.4 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 3-5% PEG 20,000, 0.1 M MES, 3% (w/v) sorbitol, 5 mM DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |