3CTR

Crystal structure of the RRM-domain of the poly(A)-specific ribonuclease PARN bound to m7GTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-03-28
Detector	MAR CCD 165 mm
Wavelength(s)	1.078
Spacegroup name	I 41 2 2
Unit cell lengths	81.107, 81.107, 78.058
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.100
R-factor	0.21124
Rwork	0.210
R-free	0.24287
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.287
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXCD
Refinement software	REFMAC (5.3.0008)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge		0.202
Number of reflections	7918
<I/σ(I)>	67.9	14.4
Completeness [%]	99.9	100
Redundancy	13.6	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	2 M LiSO4, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	2 M LiSO4, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K