3CTB
Tethered PXR-LBD/SRC-1p apoprotein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 95 |
Detector technology | IMAGE PLATE |
Collection date | 2005-05-26 |
Detector | RIGAKU RAXIS HTC |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 85.240, 89.240, 105.230 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.310 - 2.000 |
R-factor | 0.2064 |
Rwork | 0.204 |
R-free | 0.24400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nrl |
RMSD bond length | 0.011 |
RMSD bond angle | 1.149 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | MOLREP |
Refinement software | BUSTER-TNT (1.3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.039 | 0.461 |
Number of reflections | 54259 | |
<I/σ(I)> | 13.2 | 2.1 |
Completeness [%] | 98.7 | 96.9 |
Redundancy | 3.58 | 3.49 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 277 | 10-30% MPD or isopropanol, 100 mM imidazole, pH 7, VAPOR DIFFUSION, temperature 277K |