3CRL
Crystal structure of the PDHK2-L2 complex.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-16 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.413, 121.630, 71.452 |
| Unit cell angles | 90.00, 97.29, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.610 |
| R-factor | 0.223 |
| Rwork | 0.221 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.089 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | COMO |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.890 | 2.900 |
| High resolution limit [Å] | 2.610 | 2.610 |
| Rmerge | 0.039 | 0.290 |
| Number of reflections | 32860 | |
| <I/σ(I)> | 20.3 | 2.73 |
| Completeness [%] | 90.6 | 64.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 293 | 0.1 M sodium acetate (pH 5.3) and 0.5 M sodium formate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
