3CRH
Crystal structure of human fibroblast growth factor-1 with mutations Glu81Ser and Lys101Ala
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-10-10 |
Detector | RIGAKU RAXIS IIC |
Wavelength(s) | 1.541 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 77.980, 92.480, 107.540 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.970 - 2.150 |
R-factor | 0.183 |
Rwork | 0.183 |
R-free | 0.22700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.200 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.083 | 0.380 |
Number of reflections | 21461 | |
<I/σ(I)> | 6.8 | 3.13 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 4.2 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 1.5 M sodium formate and 0.4 M ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |