3CR0
Wee1 kinase complex with inhibitor PD259_809
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 2002-08-15 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 69.659, 69.659, 157.279 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.300 |
R-factor | 0.20757 |
Rwork | 0.205 |
R-free | 0.25462 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1x8b |
RMSD bond length | 0.026 |
RMSD bond angle | 2.279 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | REFMAC |
Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.250 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.118 | 0.675 |
Number of reflections | 17891 | |
<I/σ(I)> | 22.8 | 2.48 |
Completeness [%] | 99.3 | 95.1 |
Redundancy | 8.34 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | HEPES, sodium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |