3CPR
The crystal structure of Corynebacterium glutamicum dihydrodipicolinate synthase to 2.2 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 135.300, 54.810, 98.610 |
| Unit cell angles | 90.00, 129.39, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.228 |
| Rwork | 0.212 |
| R-free | 0.25900 |
| Starting model (for MR) | 1DHP was used to get an initial MR structure solution. A better MR starting solution resulted later when PDB entry 1XXX was made available for use. |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.516 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.250 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Rmerge | 0.060 | 0.167 |
| Number of reflections | 28771 | |
| <I/σ(I)> | 10.6 | |
| Completeness [%] | 96.5 | 70.9 |
| Redundancy | 3.3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | protein: 9.1 mg/mL in 20 mM Tris-HCl, pH 8.0, 0.1M KCl, 10 mM pyruvate. precipitant: 24%(w/v) PEG6000, 50 mM NaH2PO4. 10 uL droplets (5uL protein + 5 uL precipitant) suspended over 1mL of precipitant solution, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
