3CPF
Crystal structure of human eukaryotic translation initiation factor EIF5A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-03-26 |
Detector | RIGAKU RAXIS |
Wavelength(s) | 1.5418 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 76.119, 76.119, 107.752 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.996 - 2.500 |
R-factor | 0.246 |
Rwork | 0.244 |
R-free | 0.30000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1x6o |
RMSD bond length | 0.014 |
RMSD bond angle | 1.338 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 | 2.590 |
High resolution limit [Å] | 2.500 | 5.350 | 2.500 |
Rmerge | 0.076 | 0.034 | 0.964 |
Number of reflections | 11503 | ||
<I/σ(I)> | 14.3 | ||
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 12.7 | 11.7 | 12.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 22% PEG 3350, 0.2M Ammonium sulfate, 0.1M Sodium cacodylate. Cryoprotected with 20% PEG 3350 and 20% Ethylene glycol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |