3CPF

Crystal structure of human eukaryotic translation initiation factor EIF5A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-03-26
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	76.119, 76.119, 107.752
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.996 - 2.500
R-factor	0.246
Rwork	0.244
R-free	0.30000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1x6o
RMSD bond length	0.014
RMSD bond angle	1.338
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.4.0069)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	2.590
High resolution limit [Å]	2.500	5.350	2.500
Rmerge	0.076	0.034	0.964
Number of reflections	11503
<I/σ(I)>	14.3
Completeness [%]	100.0	100	100
Redundancy	12.7	11.7	12.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291	22% PEG 3350, 0.2M Ammonium sulfate, 0.1M Sodium cacodylate. Cryoprotected with 20% PEG 3350 and 20% Ethylene glycol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K