3COX

CRYSTAL STRUCTURE OF CHOLESTEROL OXIDASE COMPLEXED WITH A STEROID SUBSTRATE. IMPLICATIONS FOR FAD DEPENDENT ALCOHOL OXIDASES

Replaces:  1COX

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	67.720, 84.580, 88.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.800
R-factor	0.156
Rwork	0.156
RMSD bond length	0.011
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.800 *
Rmerge	0.066 *
Total number of observations	175309 *
Number of reflections	46364 *
Completeness [%]	97.6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	sodium/potassium phosphate	0.5-0.65 (M)
3	1	reservoir	sodium/potassium phosphate	1.3 (M)