3CO7

Crystal Structure of FoxO1 DBD Bound to DBE2 DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2007-03-06
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97949
Spacegroup name	P 32
Unit cell lengths	99.641, 99.641, 98.468
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.910
Rwork	0.267
R-free	0.27600
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.800
High resolution limit [Å]	2.700	5.800	2.700
Rmerge	0.088	0.069	0.665
Number of reflections	58591
<I/σ(I)>	11
Completeness [%]	99.6	97	99.9
Redundancy	4.3	4.3	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	277	21% PEG 4000, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG 4000
2	1	2	PEG 4000