3CO6

Crystal Structure of FoxO1 DBD Bound to DBE1 DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-10-13
Detector	ADSC QUANTUM 210
Wavelength(s)	1.00000
Spacegroup name	I 2 2 2
Unit cell lengths	65.518, 76.440, 102.143
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.100
Rwork	0.211
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.180
High resolution limit [Å]	2.100	4.520	2.100
Rmerge	0.072	0.056	0.340
Number of reflections	15307
<I/σ(I)>	12.1
Completeness [%]	99.9	99.1	100
Redundancy	4.8	4.5	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.9	277	22% PEG 4000, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG 4000
2	1	2	PEG 4000