3CMV

Mechanism of homologous recombination from the RecA-ssDNA/dsDNA structures

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Collection date	2007-08-01
Spacegroup name	P 21 21 21
Unit cell lengths	176.600, 189.800, 424.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 4.300
R-factor	0.24351
Rwork	0.243
R-free	0.26144
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.616
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	4.300
Number of reflections	87622

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		RecA4 fusion protein mixed with 2 mM AMPPNP and 10 mM MgCl2, pH 7.5, followed by crystallization from 100 mM Bis-Tris-Cl, 7% (w/v) PEG 3350, 100 mM KSCN, 25% (v/v) ethylene glycol, pH 6.5.