3CKK
Crystal structure of human methyltransferase-like protein 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-03-05 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.03310 |
Spacegroup name | P 65 |
Unit cell lengths | 90.316, 90.316, 54.940 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.550 |
R-factor | 0.19962 |
Rwork | 0.199 |
R-free | 0.21835 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vdu |
RMSD bond length | 0.009 |
RMSD bond angle | 1.276 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.610 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.068 | 0.596 |
Number of reflections | 36869 | |
<I/σ(I)> | 13.2 | 1.78 |
Completeness [%] | 99.1 | 91.3 |
Redundancy | 11.2 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 297 | 2.0 M Ammonium sulfate, 2% PEG 400, 0.1 M Na HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K |