3CKH

Crystal structure of Eph A4 receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-02-26
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 2 21 21
Unit cell lengths	53.749, 71.121, 127.004
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.710 - 2.800
R-factor	0.234
Rwork	0.228
R-free	0.28700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nuk 2bba
RMSD bond length	0.007
RMSD bond angle	1.171
Data reduction software	d*TREK
Data scaling software	d*TREK (9.9LDz)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	63.510	63.510	2.380
High resolution limit [Å]	2.300	4.950	2.300
Rmerge	0.102	0.048	0.563
Total number of observations		16598	9749
Number of reflections	21938
<I/σ(I)>	8.2	27.7	2.1
Completeness [%]	98.0	99.2	85.3
Redundancy	7.19	6.79	5.21

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	20% PEG4000, 16% isopropanol, 0.1M Hepes, pH7.5, vapor diffusion, hanging drop, temperature 298K, VAPOR DIFFUSION, HANGING DROP