3CJ5
Crystal structure of hepatitis c virus rna-dependent rna polymerase ns5b in complex with optimized small molecule fragments
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-04-10 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.91998 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 85.797, 105.735, 126.912 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | ? - 1.920 |
R-factor | 0.207 |
Rwork | 0.204 |
R-free | 0.25600 |
Refinement software | REFMAC |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 24.800 |
High resolution limit [Å] | 1.920 |
Number of reflections | 82014 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.9 | 292 | 8-11% GLYCEROL, 20-24% PEG 4000,50 mM Sodium CITRATE, pH 4.9, VAPOR DIFFUSION, temperature 292K |