3CIQ
A regulatable switch mediates self-association in an immunoglobulin fold
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 100 |
| Spacegroup name | P 1 |
| Unit cell lengths | 67.594, 68.047, 96.218 |
| Unit cell angles | 104.38, 94.15, 117.77 |
Refinement procedure
| Resolution | 50.000 - 2.900 |
| R-factor | 0.2267 |
| Rwork | 0.225 |
| R-free | 0.26393 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.895 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.079 | 0.363 |
| Number of reflections | 28565 | |
| Completeness [%] | 95.3 | 96.5 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 288 | 28% PEG 3350, 200 mM ammonium tartrate dibasic, pH 7.40, VAPOR DIFFUSION, SITTING DROP, temperature 288K |
