3CHW
Complex of Dictyostelium discoideum Actin with Profilin and the Last Poly-Pro of Human VASP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F2 |
Synchrotron site | CHESS |
Beamline | F2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-01-01 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.037, 76.183, 180.822 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.870 - 2.300 |
R-factor | 0.161 |
Rwork | 0.157 |
R-free | 0.22700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2pav |
RMSD bond length | 0.015 |
RMSD bond angle | 1.504 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.117 | 0.349 |
Number of reflections | 21716 | |
<I/σ(I)> | 20.3 | 15.8 |
Completeness [%] | 89.7 | 80.9 |
Redundancy | 11 | 11.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277.15 | 0.1M Bis-Tris, 25% PEG3350, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K |