3CH5
The crystal structure of the RanGDP-Nup153ZnF2 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.2726 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 69.700, 75.350, 123.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.760 - 2.100 |
| R-factor | 0.19821 |
| Rwork | 0.196 |
| R-free | 0.23443 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | RanGDP |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.674 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.760 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.310 | |
| Number of reflections | 18026 | |
| <I/σ(I)> | 16.1 | 6.31 |
| Completeness [%] | 98.2 | 99.9 |
| Redundancy | 4.5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 2 M ammonium sulfate, 0.1 M Bis Tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
