3CH4
The Crystal Structure of Human Phosphomavelonate Kinase At 1.8 A Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-11-29 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 93.140, 32.625, 61.786 |
Unit cell angles | 90.00, 111.01, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.760 |
R-factor | 0.199 |
Rwork | 0.199 |
R-free | 0.22100 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.523 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 8.000 | 1.800 |
High resolution limit [Å] | 1.760 | 1.760 |
Rmerge | 0.055 | 0.442 |
Number of reflections | 16987 | |
<I/σ(I)> | 16.9 | 5.4 |
Completeness [%] | 99.4 | 98.2 |
Redundancy | 9.8 | 9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 288 | 15-20% (v/v) Pentaerythritol ethoxylate (15/4 EO/OH), 15% (v/v) MPD, 0.1M Hepes, pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 288K |