3CFV
Structural basis of the interaction of RbAp46/RbAp48 with histone H4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-02 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 108.661, 44.790, 109.592 |
| Unit cell angles | 90.00, 90.71, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.600 |
| R-factor | 0.20178 |
| Rwork | 0.199 |
| R-free | 0.25675 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | our Rabp46/h4 SAD data phasing model |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.865 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.143 | 0.731 |
| Number of reflections | 33119 | |
| <I/σ(I)> | 18.5 | 3.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 277 | 13% PEG 2000, 0.2M Ca acetate, 0.1M Na Cacodylate, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
