3CFS
Structural basis of the interaction of RbAp46/RbAp48 with histone H4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.665, 85.725, 117.723 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.530 - 2.400 |
| R-factor | 0.18745 |
| Rwork | 0.184 |
| R-free | 0.24862 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SAD data phased model |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.750 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.500 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.130 | 0.468 |
| Number of reflections | 18615 | |
| <I/σ(I)> | 16 | 1.9 |
| Completeness [%] | 99.8 | 98 |
| Redundancy | 7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 277 | 18% PEG8000, 0.2M Ca acetate, 0.1M Na Cacodylate, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
