3CFC
High-resolution structure of blue fluorescent antibody EP2-19G2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-24 |
Detector | MARMOSAIC 325 mm CCD |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 87.891, 87.891, 114.111 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.760 - 1.700 |
R-factor | 0.193 |
Rwork | 0.191 |
R-free | 0.23100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fl3 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.609 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.3.0011) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 49748 | |
<I/σ(I)> | 45.4 | 2.8 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.5 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.5 | 18% PEG 8000, 0.1M ACETATE, PH 4.5, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 298K, pH 4.50 |