1FL3

CRYSTAL STRUCTURE OF THE BLUE FLUORESCENT ANTIBODY (19G2) IN COMPLEX WITH STILBENE HAPTEN AT 277K

Summary for 1FL3

• Entry DOI: 10.2210/pdb1fl3/pdb
• Descriptor: BLUE FLUORESCENT ANTIBODY (19G2)-HEAVY CHAIN, BLUE FLUORESCENT ANTIBODY (19G2)-LIGHT CHAIN, 4-(4-STYRYL-PHENYLCARBAMOYL)-BUTYRIC ACID, ... (4 entities in total)
• Functional Keywords: immunoglobulin fold, immune system
• Biological source: Mus musculus (house mouse); More
• Total number of polymer chains: 4
• Total formula weight: 91181.90

Authors

Stevens, R.C.,Beuscher IV, A.E. (deposition date: 2000-08-11, release date: 2000-11-01, Last modification date: 2024-10-30)

Primary citation

Simeonov, A.,Matsushita, M.,Juban, E.A.,Thompson, E.H.,Hoffman, T.Z.,Beuscher IV, A.E.,Taylor, M.J.,Wirsching, P.,Rettig, W.,McCusker, J.K.,Stevens, R.C.,Millar, D.P.,Schultz, P.G.,Lerner, R.A.,Janda, K.D.
Blue-fluorescent antibodies.
Science, 290:307-313, 2000

PubMed Abstract: The forte of catalytic antibodies has resided in the control of the ground-state reaction coordinate. A principle and method are now described in which antibodies can direct the outcome of photophysical and photochemical events that take place on excited-state potential energy surfaces. The key component is a chemically reactive optical sensor that provides a direct report of the dynamic interplay between protein and ligand at the active site. To illustrate the concept, we used a trans-stilbene hapten to elicit a panel of monoclonal antibodies that displayed a range of fluorescent spectral behavior when bound to a trans-stilbene substrate. Several antibodies yielded a blue fluorescence indicative of an excited-state complex or "exciplex" between trans-stilbene and the antibody. The antibodies controlled the isomerization coordinate of trans-stilbene and dynamically coupled this manifold with an active-site residue. A step was taken toward the use of antibody-based photochemical sensors for diagnostic and clinical applications.

PubMed: 11030644
DOI: 10.1126/science.290.5490.307

Experimental method

X-RAY DIFFRACTION (2.45 Å)