3CF5

Thiopeptide antibiotic Thiostrepton bound to the large ribosomal subunit of Deinococcus radiodurans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 300 mm CCD
Spacegroup name	I 2 2 2
Unit cell lengths	169.900, 408.900, 694.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 3.300
R-factor	0.276
Rwork	0.276
R-free	0.31800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zjr
RMSD bond length	0.010
RMSD bond angle	1.550
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.360
High resolution limit [Å]	3.300	3.300
Number of reflections	342617
Completeness [%]	94.1	84.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				VAPOR DIFFUSION, HANGING DROP
1				VAPOR DIFFUSION, HANGING DROP