3CF5
Thiopeptide antibiotic Thiostrepton bound to the large ribosomal subunit of Deinococcus radiodurans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 300 mm CCD |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 169.900, 408.900, 694.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 3.300 |
R-factor | 0.276 |
Rwork | 0.276 |
R-free | 0.31800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zjr |
RMSD bond length | 0.010 |
RMSD bond angle | 1.550 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.360 |
High resolution limit [Å] | 3.300 | 3.300 |
Number of reflections | 342617 | |
Completeness [%] | 94.1 | 84.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | |||
1 | VAPOR DIFFUSION, HANGING DROP |