3CF0
Structure of D2 subdomain of P97/VCP in complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 173.317, 167.223, 209.465 |
| Unit cell angles | 90.00, 112.29, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.000 |
| R-factor | 0.244 |
| Rwork | 0.243 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.078 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 108870 | |
| Completeness [%] | 98.1 | 99.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 298 | 100 mM Tris, pH 7.1, 2.45 M ammonium sulfate, and 0.2 M lithium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
