3CEH
Human liver glycogen phosphorylase (tense state) in complex with the allosteric inhibitor AVE5688
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Spacegroup name | P 31 |
| Unit cell lengths | 124.115, 124.115, 122.491 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.600 - 2.800 |
| R-factor | 0.17 |
| Rwork | 0.165 |
| R-free | 0.24700 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.599 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 43.600 |
| High resolution limit [Å] | 2.800 |
| Number of reflections | 51974 |
| Completeness [%] | 99.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 7 mg/ml in 20 mM BES, 1 mM EDTA, 0.5 mM DTT, pH 6.5, 50 mM NBG, 2.5 mM AVE5688, VAPOR DIFFUSION, HANGING DROP |
