3CDG
Human CD94/NKG2A in complex with HLA-E
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-02 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | I 41 3 2 |
Unit cell lengths | 345.201, 345.201, 345.201 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.400 |
R-factor | 0.25 |
Rwork | 0.249 |
R-free | 0.27900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | pbd code 3bdw 3bze |
RMSD bond length | 0.006 |
RMSD bond angle | 0.944 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 3.400 | 3.400 |
Number of reflections | 48105 | |
<I/σ(I)> | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 291 | Ammonium sulfate, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 291K |