3CC0
The Dvl2 PDZ Domain in Complex with the N3 Inhibitory Peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-09 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 65 |
Unit cell lengths | 52.617, 52.617, 171.098 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.750 |
R-factor | 0.19 |
Rwork | 0.188 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3cbx |
RMSD bond length | 0.014 |
RMSD bond angle | 1.458 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.058 | 0.436 |
Number of reflections | 26928 | |
<I/σ(I)> | 19.8 | 3.5 |
Completeness [%] | 99.5 | 99.4 |
Redundancy | 3.8 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 292 | 1.0 M Ammonium citrate tribasic, 0.1 M BIS-TRIS propane, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 292K |