3CBB
Crystal Structure of Hepatocyte Nuclear Factor 4alpha in complex with DNA: Diabetes Gene Product
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-08-20 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.92017 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 121.628, 35.425, 70.985 |
Unit cell angles | 90.00, 119.36, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
Rwork | 0.213 |
R-free | 0.25200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.031 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.064 | 0.304 |
Number of reflections | 17094 | |
<I/σ(I)> | 12.1 | |
Completeness [%] | 93.9 | 72.4 |
Redundancy | 5.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 298 | 26% PEG 4000, 80mM Magnesium acetate, 50mM Sodium citrate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 4000 | ||
2 | 1 | 1 | Magnesium acetate | ||
3 | 1 | 1 | Sodium citrate | ||
4 | 1 | 2 | PEG 4000 | ||
5 | 1 | 2 | Magnesium acetate | ||
6 | 1 | 2 | Sodium citrate |