3CB2
Crystal structure of human gamma-tubulin bound to GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-12-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.744, 108.755, 83.347 |
Unit cell angles | 90.00, 96.33, 90.00 |
Refinement procedure
Resolution | 82.760 - 2.303 |
R-factor | 0.18908 |
Rwork | 0.187 |
R-free | 0.23786 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB 1Z5V stripped of sidechains and ligands |
RMSD bond length | 0.008 |
RMSD bond angle | 1.149 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 82.760 | 2.380 |
High resolution limit [Å] | 2.303 | 2.303 |
Number of reflections | 38587 | |
Completeness [%] | 98.9 | 97 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.2 | 298 | top solution: 83 mM HEPES pH 7.2, 1.2M KCl, 20% PEG6000, 1mM GDP; reservoir: 83mM HEPES pH 7.2, 0.6M KCl, 20% PEG6000, 1 mM GDP. Protein was mixed 1:1 or 1:2 with top solution, and equilibrated over the reservoir solution., VAPOR DIFFUSION, temperature 298K |