3C6W
Crystal structure of the ING5 PHD finger in complex with H3K4me3 peptide
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-22 |
Detector | NOIR-1 |
Wavelength(s) | 1.2574, 1.28304, 1.28264 |
Spacegroup name | P 43 |
Unit cell lengths | 68.140, 68.140, 28.250 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.000 - 1.750 |
R-factor | 0.186 |
Rwork | 0.186 |
R-free | 0.19900 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.337 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.000 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.070 | 0.219 |
Number of reflections | 13176 | |
<I/σ(I)> | 17.7 | 6.2 |
Redundancy | 6.95 | 5.23 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291.15 | 1.6 M Sodium Citrate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291.15K |