3C5X
Crystal structure of the precursor membrane protein- envelope protein heterodimer from the dengue 2 virus at low pH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 71.209, 108.595, 108.738 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.2555 |
| Rwork | 0.253 |
| R-free | 0.27530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tg8 |
| RMSD bond length | 0.027 |
| RMSD bond angle | 1.727 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.056 | 0.408 |
| Number of reflections | 42500 | |
| <I/σ(I)> | 30.3 | 3.8 |
| Completeness [%] | 98.5 | 92.9 |
| Redundancy | 6.8 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | Bis-Tris 0.1M pH5.5, PEG 3350 12%, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
