3C4B
Structure of RNaseIIIb and dsRNA binding domains of mouse Dicer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 66.496, 66.496, 134.081 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.680 |
| R-factor | 0.1814 |
| Rwork | 0.180 |
| R-free | 0.21036 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.406 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.724 |
| High resolution limit [Å] | 1.660 | 1.680 |
| Rmerge | 0.060 | 0.444 |
| Number of reflections | 30584 | |
| <I/σ(I)> | 31.7 | 2.27 |
| Completeness [%] | 90.0 | 56.55 |
| Redundancy | 9.3 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 20-26% PEG400, 100 mM HEPES pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 20-26% PEG400, 100 mM HEPES pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
