3C4B

Structure of RNaseIIIb and dsRNA binding domains of mouse Dicer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-02-15
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9795
Spacegroup name	P 42 21 2
Unit cell lengths	66.496, 66.496, 134.081
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 1.680
R-factor	0.1814
Rwork	0.180
R-free	0.21036
RMSD bond length	0.014
RMSD bond angle	1.406
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.4.0067)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.724
High resolution limit [Å]	1.660	1.680
Rmerge	0.060	0.444
Number of reflections	30584
<I/σ(I)>	31.7	2.27
Completeness [%]	90.0	56.55
Redundancy	9.3	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	20-26% PEG400, 100 mM HEPES pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	7	295	20-26% PEG400, 100 mM HEPES pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K