3C3O

ALIX Bro1-domain:CHMIP4A co-crystal structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97607
Spacegroup name	C 1 2 1
Unit cell lengths	120.668, 62.681, 76.124
Unit cell angles	90.00, 122.13, 90.00

Refinement procedure

Resolution	32.220 - 2.150
R-factor	0.21868
Rwork	0.214
R-free	0.28403
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	2oew.pdb
RMSD bond length	0.019
RMSD bond angle	1.710
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	REFMAC
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.230
High resolution limit [Å]	2.150	2.150
Number of reflections	25024
Completeness [%]	94.9	75.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	15% PEG 8,000, 100mM Na MES pH 6.5, 200mM Na Acetate, VAPOR DIFFUSION, SITTING DROP, temperature 277K
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	15% PEG 8,000, 100mM Na MES pH 6.5, 200mM Na Acetate, VAPOR DIFFUSION, SITTING DROP, temperature 277K