3C20
Crystal Structure of Threonine-sensitive Aspartokinase from Methanococcus jannaschii with L-aspartate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-10 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.039 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 107.973, 199.071, 96.394 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.700 |
| R-factor | 0.23976 |
| Rwork | 0.238 |
| R-free | 0.27298 |
| Starting model (for MR) | PDB code:3C1M |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.183 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.770 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 30974 | |
| <I/σ(I)> | 28.3 | 3 |
| Completeness [%] | 0.9 | |
| Redundancy | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 14% PEG4000, 0.1M Tris, 0.8M ammonium formate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
