3C1B

The effect of H3 K79 dimethylation and H4 K20 trimethylation on nucleosome and chromatin structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	292
Detector technology	CCD
Collection date	2006-10-20
Spacegroup name	P 21 21 21
Unit cell lengths	105.670, 109.830, 181.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.000 - 2.200
Rwork	0.240
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1aoi
RMSD bond length	0.006
RMSD bond angle	1.247
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.066	0.508
Number of reflections	106458
<I/σ(I)>		2.6
Completeness [%]	99.0	99
Redundancy		99

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	292	Manganese chloride, Potassium chloride, Potassium cacodylate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 292K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	Manganese chloride
2	1	1	Potassium cacodylate
3	1	2	Potassium cacodylate
4	1	2	Potassium chloride