3C1B
The effect of H3 K79 dimethylation and H4 K20 trimethylation on nucleosome and chromatin structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 292 |
| Detector technology | CCD |
| Collection date | 2006-10-20 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 105.670, 109.830, 181.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.000 - 2.200 |
| Rwork | 0.240 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aoi |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.247 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.066 | 0.508 |
| Number of reflections | 106458 | |
| <I/σ(I)> | 2.6 | |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 292 | Manganese chloride, Potassium chloride, Potassium cacodylate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Manganese chloride | ||
| 2 | 1 | 1 | Potassium cacodylate | ||
| 3 | 1 | 2 | Potassium cacodylate | ||
| 4 | 1 | 2 | Potassium chloride |
