3C02
X-ray structure of the aquaglyceroporin from Plasmodium falciparum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.11587 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 92.430, 92.430, 193.168 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.200 - 2.050 |
| R-factor | 0.18175 |
| Rwork | 0.181 |
| R-free | 0.19824 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fx8 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.628 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.200 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.084 | 0.478 |
| Number of reflections | 24719 | |
| <I/σ(I)> | 28.8 | 2.25 |
| Completeness [%] | 95.5 | 69.9 |
| Redundancy | 15 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 291 | 17% PEG MME 2000, 0.1M Sodium citrate, 15% Glycerol, 0.04M n-octyl-B-D-glucopyranoside, 0.01 Nickel sulfate, 0.01M DTT, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
