3BXJ
Crystal Structure of the C2-GAP Fragment of synGAP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 61 |
| Unit cell lengths | 113.320, 113.320, 166.090 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.970 - 3.000 |
| R-factor | 0.246 |
| Rwork | 0.244 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.551 |
| Data scaling software | XSCALE |
| Phasing software | EPMR (2.5) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.970 | 3.170 | |
| High resolution limit [Å] | 3.000 | 20.000 | 3.000 |
| Rmerge | 0.067 | 0.383 | |
| Number of reflections | 32876 | 3680 | |
| <I/σ(I)> | 22.52 | 5.1 | |
| Completeness [%] | 99.6 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 4.9 | 298 | 80 mM ammonium sulfate, 11% PEG 3000, 0.1 M sodium phosphate/citrate, 0.1mM EDTA as additive, pH 4.9, hanging drop, temperature 298K |
