3BUP
Golgi alpha-mannosidase II D341N acid-base catalyst mutant with bound mannose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-06-15 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.670, 109.486, 138.584 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.790 - 2.030 |
| R-factor | 0.154 |
| Rwork | 0.152 |
| R-free | 0.19500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hty |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.344 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.080 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.068 | 0.344 |
| Number of reflections | 66283 | |
| <I/σ(I)> | 11.6 | 4.1 |
| Completeness [%] | 96.0 | 79.8 |
| Redundancy | 7.8 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Tris, NaCl, PEG6000, MPD. Crystal soaked in PNP-mannose at 4C, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
