3BT1
Structure of urokinase receptor, urokinase and vitronectin complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 97.353, 105.187, 55.360 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.680 - 2.800 |
R-factor | 0.244 |
Rwork | 0.241 |
R-free | 0.30800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fd6 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.821 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.081 | 0.803 |
Number of reflections | 14540 | |
<I/σ(I)> | 33.1 | 2.6 |
Completeness [%] | 99.5 | 100 |
Redundancy | 5.9 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 7.5 | 295 | 12% PEG 3350, 50mM HEPES pH 7.5, MICRODIALYSIS, temperature 295K |