3BSZ
Crystal structure of the transthyretin-retinol binding protein-Fab complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-10-21 |
Detector | MAR scanner 180 mm plate |
Wavelength(s) | 1.3 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 159.620, 223.180, 121.430 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.000 | 3.510 |
High resolution limit [Å] | 3.360 | 3.360 |
Rmerge | 0.160 | 0.340 |
Number of reflections | 25746 | |
<I/σ(I)> | 3.3 | 1.9 |
Completeness [%] | 84.4 | 77.7 |
Redundancy | 3 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 295 | 2.35M ammonium phosphate, 10mM sodium citrate, 10mM beta-mercaptoethanol, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |