3BS9
X-ray structure of human TIA-1 RRM2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2006-08-16 |
Detector | BRUKER PROTEUM |
Spacegroup name | P 61 |
Unit cell lengths | 56.500, 56.500, 76.700 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.950 |
Rwork | 0.216 |
R-free | 0.24540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | N-terminal RRM of poly-A binding protein PDB entry 1CVJ |
Data reduction software | PROTEUM PLUS (PLUS) |
Data scaling software | PROTEUM PLUS (PLUS) |
Phasing software | PHASER |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 24.460 |
High resolution limit [Å] | 1.950 |
Number of reflections | 10137 |
Completeness [%] | 99.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 1:1 mixture of protein with 15% polyethylene glycol 3350, 0.5 M KI, 0.1 M Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |