3BRW

Structure of the Rap-RapGAP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2006-07-13
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97916
Spacegroup name	P 31 2 1
Unit cell lengths	209.723, 209.723, 108.217
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.080 - 3.400
R-factor	0.23624
Rwork	0.234
R-free	0.28043
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1SRQ for Rap1GAP
RMSD bond length	0.008
RMSD bond angle	1.128
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	182.570	3.500
High resolution limit [Å]	3.400	3.400
Rmerge	0.087	0.335
Number of reflections	37639
<I/σ(I)>	15.48	3.6
Completeness [%]	99.2	98.4
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	285	8-11% PEG 2000 MME, 100mM MES pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K